## Abstract A sensitive and reproducible realβtime PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an βinβhouseβ qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B pati
β¦ LIBER β¦
A quantitative, internally controlled real-time PCR Assay for the detection of parvovirus B19 DNA
β Scribed by Claudia Aberham; Claudia Pendl; Patricia Gross; Gerold Zerlauth; Matthias Gessner
- Book ID
- 114139618
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 100 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0166-0934
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## Abstract A realβtime PCR assay was developed for quantitative detection of B19 DNA in clinical serum samples. The assay was carried out using a LightCycler instrument and product formation was monitored continuously with the fluorescent doubleβstranded DNA binding dye SYBR Green I. With an optim