A Quantitative Determination of Singlet Oxygen with Horseradish Peroxidase

✍ Scribed by M. Kreitner; G. Alth; H. Koren; S. Loew; R. Ebermann

• Book ID: 102562343
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 454 KB
• Volume: 213
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1386

✦ Synopsis

A singlet oxygen determination method based on the formation of dimethyl-(2,5)-2-methoxy-5-hydroperoxydihydrofuran (\left(\mathrm{DMFO}{2}\right.) ) followed by acid hydrolysis with (0.1 \mathrm{~N} \mathrm{H}{2} \mathrm{SO}{4}) is described. Hydrogen peroxide is formed thereby as a product of hydrolysis which is then determined by the horseradish peroxidase-catalyzed formation of the homovanillic acid dimer. Since (\mathrm{DMFO}{2}) is a crystalline compound with sufficient stability it can be used as a primary standard for the determination. The peroxidase-catalyzed hydrogen peroxide determination allows detection of singlet oxygen concentrations as low as (10^{-7} \mathrm{M}). 1983 Academic Press, Inc.