A Quantitative Comparison of Wild-Type and Gatekeeper Mutant Cdk2 for Chemical Genetic Studies with ATP Analogues

A colorimetric assay for a pyridoxal pho

A colorimetric assay for a pyridoxal phosphate-dependent β-replacement reaction with l-cysteine: Application to studies of wild-type and mutant tryptophan synthase α2β2 complexes

✍ Arvind M. Kayastha; Edith Wilson Miles 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 428 KB

We present an improved and simple direct assay for formation of inorganic sulfide from L-cysteine in a beta-replacement reaction catalyzed by tryptophan synthase. This method provides a useful enzymatic assay for pyridoxal phosphate-dependent beta-replacement reactions in which the amino acid substr