✦ LIBER ✦
A quantitative comparison of synaptic density following perfusion versus immersion fixation in the rat cerebral cortex
✍ Scribed by Marie-H. Monfils; Doug F. Bray; Ira Driscoll; Jeffrey A. Kleim; Bryan Kolb
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 353 KB
- Volume
- 67
- Category
- Article
- ISSN
- 1059-910X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The main objective of the present study was to perform an unbiased comparison of immersion vs. perfusion techniques to assess whether we could use the former to quantify synapses through electron microscopy (EM). Using the immersion technique is ideally suited for instances in which the specimen under study could not be perfused under the standard EM protocol. Our results suggest that, despite suboptimal qualitative results, fixation by immersion allows for adequate quantification of synapses. Microsc. Res. Tech. 67:300–304, 2005. © 2005 Wiley‐Liss, Inc.