A quantitative autoradiographic study of125I atrial natriuretic factor in the heart of a teleost fish (Conger conger)
✍ Scribed by Cerra, M. C. ;Canonaco, M. ;Tota, B.
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 489 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Quantitative receptor autoradiographic study of ^125^I‐atrial natriuretic peptide factor (ANF) in the heart of a teleost fish Conger conger has shown that a heterogenous distribution of ^125^I‐ANF binding exists in the different cardiac regions. Elevated ANF binding densities (3,790 fmol/mg protein) were encountered in the innermost layer (tunica intima) of the bulbus arteriosus while lower binding levels (293–403 fmol/mg protein) were revealed in atrium and ventricle. In order to determine ^125^I‐ANF binding characteristics (K~D~, B~max~) in the above cardiac sites, saturation binding assays were carried out. The results show that low ^125^I‐ANF K~D~ values (28.8–52.6 pM) were found in the atrium and in the bulbus arteriosus with respect to the higher K~D~ values (373 pM) of the ventricle. The number of binding sites were respectively 632 and 1,279 fmol/mg protein for the atrium and the ventricle, while a substantially elevated B~max~ of 7,235 fmol/mg protein was found for the bulbus arteriosus. These results may furnish some insights concerning ANF receptor binding activity and its putative regulatory role of different cardiac functions. © 1992 Wiley‐Liss, Inc.