A quantitative approach to the study of the adsorption/desorption of cellulase components in a crude cellulase mixture

✍ Scribed by Alex H. C. Yu; Dora Lee; John N. Saddler

• Book ID: 104644714
• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 446 KB
• Volume: 7
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00152618

✦ Synopsis

Chromatofocusing

was used to separate and identify cellulase components for the study of their adsolptionldesorption onto lignocellulosic substrates during cellulose hydrolysis. The separated cellulase components were characterized with respect to their M.W.s and enzymatic activities. Adsorption of the cellulase components onto five different cellulosic substrates was quantified. All the major cellulase components adsorbed to all the substrates studied with only minor differences observed in the amount of binding of each cellulase component.