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A qRT-PCR-based method for the measurement of rrn operon copy number

โœ Scribed by T. Zhang; M.-F. Shao; H.H.-P. Fang

Book ID
111192352
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
97 KB
Volume
49
Category
Article
ISSN
0266-8254

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โœ Naoya Hosono; Michiaki Kubo; Yumiko Tsuchiya; Hiroko Sato; Takuya Kitamoto; Susu ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 605 KB

We report the development of a real-time Invader assay combined with multiplex PCR (mPCR-RETINA), an SNP-based approach that can measure the allelic ratio in copy number variation (CNV) regions of a genome. RETINA monitors the real-time fluorescence intensity of each allele during the Invader assay