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A Putative Role for the Immunoproteasome in the Maintenance of Pluripotency in Human Embryonic Stem Cells

✍ Scribed by Stuart P. Atkinson; Joseph Collin; Neganova Irina; George Anyfantis; Bo Kim Kyung; Majlinda Lako; Lyle Armstrong

Book ID: 112211922
Publisher: AlphaMed Press
Year: 2012
Tongue: English
Weight: 538 KB
Volume: 30
Category: Article
ISSN: 1066-5099
DOI: 10.1002/stem.1113

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## Abstract Realizing the potential clinical and industrial applications of human embryonic stem cells (hESCs) is limited by the need for costly, labile, or undefined growth substrates. Here we demonstrate that trypsin passaging of the hESC lines, HUES7 and NOTT1, on oxygen plasma etched tissue cul