𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Protease Assay via Precolumn Derivatization and High-Performance Liquid Chromatography

✍ Scribed by S.L. Zuo; Q. Guo; Y.H. Chang

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
292 KB
Volume
222
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Amino acid analysis using 1-naphthylisoc
Amino acid analysis using 1-naphthylisocyanate as a precolumn high performance liquid chromatography derivatization reagent
✍ Amos Neidle; Miriam Banay-Schwartz; Shirley Sacks; David S. Dunlop πŸ“‚ Article πŸ“… 1989 πŸ› Elsevier Science 🌐 English βš– 550 KB

A method which uses 1-naphthylisocyanate as an HPLC precolumn derivatization reagent for amino acid analysis is described. Derivatization is carried out by adding the isocyanate dissolved in dry acetone to a buffered amino acid solution followed by extraction of the excess reagent with cyclohexane.

A fluorometric assay for HIV-protease ac
A fluorometric assay for HIV-protease activity using high-performance liquid chromatography
✍ Paul P. Tamburini; Robert N. Dreyer; Jutta Hansen; Jack Letsinger; Jim Elting; A πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 883 KB

A rapid sensitive method for the quantification of in vitro HIV-protease activity has been developed on the basis of the endoproteolytic conversion of N-Dns-SQ-NYPIV to N-Dns-SQNY. The use of the N-dansyl group as a fluorescence label was shown to not significantly alter the apparent kinetic paramet

High-performance liquid chromatography w
High-performance liquid chromatography with sequential injection for online precolumn derivatization of some heavy metals
✍ Rodjana Burakham; Supalax Srijaranai; Kate Grudpan πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 727 KB

## Abstract HPLC was coupled with sequential injection (SI) for simultaneous analyses of some heavy metals, including Co(II), Ni(II), Cu(II), and Fe(II). 2‐(5‐Nitro‐2‐pyridylazo)‐5‐[__N__‐propyl‐__N__‐(3‐sulfopropyl)amino]phenol (nitro‐PAPS) was employed as a derivatizing reagent for sensitive spec