A radiochemical microassay for soluble and membrane-bound glutamate decarboxylase (GAD) is described. Up to 180 samples can be determined per day with a variation coefficient of 2%. The method detects newly synthesized gamma-amino-n-butyric acid in the picomole range and can easily be applied to oth
✦ LIBER ✦
A proposed structure for the 330-nm chromophore of glutamate decarboxylase and other pyridoxal 5′-phosphate dependent enzymes
✍ Scribed by Marion H. O'Leary
- Book ID
- 115732678
- Publisher
- Elsevier Science
- Year
- 1971
- Weight
- 435 KB
- Volume
- 242
- Category
- Article
- ISSN
- 0005-2744
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The pyridoxal-5Ј-phosphate-dependent enzymes (B 6 enzymes) are grouped into three main families named ␣, , and ␥. Proteins in the ␣ and ␥ families share the same fold and might be distantly related, while those in the  family exhibit specific structural features. The rat aromatic L-amino acid deca