A program in BASIC for the determination of molecular weights and linear graphic representation of an electrophoretic protein pattern: Application to serotypes A and B of Candida albicans studied by polyacrylamide gel electrophoresis and immunoblotting
✍ Scribed by Dr. OumaïMa Ibrahim-Granet; Jean M. Delga; Christian Granet; Edouard Drouhet
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 708 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
A program in BASIC for the determination of molecular weights and linear graphic representation of an electrophoretic protein pattern: Application to serotypes A and B of Candida albicans studied by polyacrylamide gel electrophoresis and immunoblotting
A program in BASIC Microsoft for a microcomputer is described for the analysis of electrophoretic patterns obtained by polyacrylamide gel electrophoresis. The program enables a rapid calculation of relative molecular masses and designs three types of graphs: the protein pattern, the linear representation of molecular masses and the linear regression curve calculated from several standard proteins. The application of this program for the study of serotypes A and B of the yeast Cundida albicans is shown. 1 Introduction Electrophoretic separation of proteins is a well-established technique in clarifying variations within or between fungal species. Many fungi have been studied taxonomically by electrophoresis including dermatophytes 111, Cundida 121, Fonsecaea pedrosoi [31 and others. At present other authors use computer facilities for the analysis of the data from scanning densitometers [4, 51. This paper describes a program written in Microsoft BASIC for the analysis of electrophoretic patterns obtained by polyacrylamide gel electrophoresis (PAGE). The application of the program, which can be implemented on a Texas Instrument Personal Computer (TIPC) to the study of Candida albicuns serotypes A and B is shown in this report.* 2 Material and methods
2.1 TIPC hardware characteristics
Ram installed: 192 kbytes; central processor unit: 16 bit 8088; numeric coprocessor: 8087; 2 floppy disks: 320 kbytes; option installed: 3 planes graphics; operating system: MS-Dos version 2-1 1.
2.2 Program description
The program suite consists of five programs: MENU 1.00, STORE 1.00, VERIFY 1.00, CALCUL 1.00 and GRAPH 1.00. "MENU" provides the means for selecting one of the following programs. "STORE" files all the data of one experiment. For a given protein pattern the data were obtained by measuring manually the electrophoretic mobility of each frac-* We would be willing to copy this program onto a compatible floppy disk for anyone sending an appropriate disk. Such a disk could only be used with a TIPC microcomputer. For any other type of computer using the Microsoft BASIC. the published program can be entered manually.