A private idiotype can become recurrent through genetic recombination and gene(s) unlinked to the Igh locus governs its expression
✍ Scribed by Frederic Mertens; Claudia Berek; Fabienne Andris; Fabienne Willems; Maryse Brait; Robert Miller; Roy J. Riblet; Moncef Slaoui; Jacques Urbain
- Book ID
- 102823286
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 910 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Any immune response is characterized by its idiotypic profile.Two different kinds of idiotype (Id) have been described. Private Id are restricted to a few individuals from a species while recurrent Id appear in a large majority of individuals from the same species immunized with the same antigen. We describe, in this report, an experimental model whereby a private Id can become recurrent through genetic recombination. The immune response of A mice against the hapten arsonate is characterized by a recurrent Id called cross-reactive idiotype A (CRIA). A strongly CRI, called CRIA-like, can be occasionally detected in some BALBk mice (5% to 10%) immunized with arsonate. Molecular studies show that CRIA and CRIA-like antibodies have highly homologous D segments and identical light chains. By contrast, their VH segments are vastly dissimilar. We have examined the anti-arsonate response of inbred strains of mice whose Igh loci are recombinant between those of A/He and BALB/c. Interestingly, we have observed that the CRIA-like Id which is private in BALB/c becomes recurrent in the AXC-1 strain which harbors theVH genes from BALBk, the DH and CH genes from A/He. Structural studies demonstrate that highly homologous,VH,VL and D segments are used in BAL,B/c and AXC-lmice. The basis for this differential expression of highly similar genes could be linked to the DH locus. However, F1 mice stemming from the cross between AXC-1 and BALBk do not express the Id. The backcross analysis shows that the non-expression of the Id in F1 mice depends on genes unlinked to the Igh locus.