A practical approach to the implementation of selectivity in homonuclear multidimensional NMR with frequency selective-filtering techniques. Application to the chemical structure elucidation of complex oligosaccharides
✍ Scribed by Christian Roumestand; Corinne Delay; Jose A. Gavin; Daniel Canet
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 466 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0749-1581
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✦ Synopsis
This review describes the use of frequency-selective filters for the design of selective NMR experiments. Frequency-selective filters constitute 'user-friendly' and efficient alternatives to selective excitation in most applications. Different selective schemes are discussed, which are based either on selective inversion or selective refocusing of the frequencies of interest. Selective filters can be used to transform 2D (or 3D) experiments into 1D (or 2D) analogues, or to restrict the matrix size in higher dimensional spectra. Accurate NMR parameters can be easily extracted from such spectra in short measuring times, with high digital resolution and minimum data storage. For these reasons, these experiments are a very useful alternative to conventional multidimensional experiments in structural and/or conformational studies of small-and medium-sized molecules. Moreover, using DANTE procedures for selectivity, they can be easily implemented on 'routine' spectrometers, without the need for sophisticated hardware. These methods have been tested on a complex heterosidic compound, where they prove to afford outstanding interest in achieving proton resonance assignment and chemical structure delineation.