A micromethod for the determination of c
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Daniel J. Strydom
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Article
📅
1988
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Elsevier Science
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English
⚖ 616 KB
Proteins and peptides are digested with anhydrous hydrazine and the free amino acids, released from their carboxyl termini, are analyzed by reverse-phase chromatography as phenylthiocarbamyl (PTC) derivatives. Most of the potentially interfering hydrazides are removed due to the insolubility of thei