A possible role of autogenous IFN-β for cytokine productions in human fibroblasts

✍ Scribed by Emiko Sano; Kensaku Ohashi; Yuichiro Sato; Masamitsu Kashiwagi; Atsuhiro Joguchi; Norio Naruse

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 669 KB
• Volume: 100
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.21128

✦ Synopsis

Abstract

It has been already known that human diploid fibroblasts are able to produce not only high levels of IFN‐β but also various kinds of cytokines by poly rI: poly rC, and some inflammatory cytokines are induced by IFN‐β gene activation. We also obtained similar results. However, in our system, cytokine productions were extremely enhanced by treating the cells with a low dose of type 1 IFN and the priming effects on cytokine productions were blocked by cycloheximide similar to those on IFN‐β productions. Most of cytokines were produced later than IFN‐β and synthesis patterns of their mRNA showed the same phenomena. We made clear that cytokine productions by poly rI: poly rC are mediated by secreted IFN‐β at a protein level using a monoclonal antibody against human IFN‐β. Further, it was shown that intra‐cellular IFN‐β which is not secreted might also participate in cytokine productions. Meanwhile, IL‐1β induced various kinds of cytokines in human fibroblasts and production time courses of these cytokines were similar to those of poly rI: poly rC induced cytokines. Although secreted IFN‐β was not detected in IL‐1β stimulated culture, expression of IFN‐β mRNA was augmented. These results showed that priming effects of type 1 IFN on cytokine productions by poly rI: poly rC might not be the direct action, but successive IFN‐β production might be essential in the production processes of other cytokines. Further, it was suggested that inducible IFN‐β might also take part in IL‐1β‐induced cytokine productions. J. Cell. Biochem. 100: 1459–1476, 2007. © 2006 Wiley‐Liss, Inc.