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A possible role for the pcnB gene product of Escherichia coli in modulating RNA:RNA interactions

โœ Scribed by Masters, Millicent ;March, John B. ;Oliver, I. R. ;Collins, J. F.

Publisher
Springer
Year
1990
Tongue
English
Weight
377 KB
Volume
220
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

The sequence of the PcnB protein of Escherichia coli, a protein required for copy number maintenance of ColE1-related plasmids, was compared with the PIR sequence database. Strong local similarities to the sequence of the E. coli protein tRNA nucleotidyltransferase were found. Since a substrate of the latter protein, tRNA, structurally resembles the RNAs that control ColE1 copy number we believe that we may have identified a region in PcnB that interacts with these RNAs. Consistent with this idea is our observation that PcnB is required for the replication of R1, a plasmid whose replication is also regulated by a small RNA.

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