A possible effect of the Na+ concentration in oviductal fluid on amino acid uptake by cleavage-stage mouse embryos
✍ Scribed by van Winkle, Lon J. ;Campione, Allan L. ;Kester, Samara E.
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 412 KB
- Volume
- 235
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Two-and four-cell mouse embryos exhibited both Na+dependent and Na+-independent components of zwitterionic a-amino acid transport, which we tentatively ascribe to the A and L amino acid transport systems, respectively. Uptake of taurine was virtually all Na f -dependent and is probably via the / 3 system. Naf-independent L -1ysine uptake by two-cell embryos may have been via system y+. The small amount of lysine transport which was Naf-dependent (30% of the total) could not be attributed to any well known transport system and may have been due to the early ontogenetic expression of a newly described transport system which predominates in preimplantation blastocysts. We conclude that the rate of Naf -dependent amino acid transport in two-cell mouse embryos could be significantly affected in situ by changes in the "af] which are known to occur in oviductal fluid.