✦ LIBER ✦
A Positive Selection Vector Combining Tetracycline Resistance That Eliminates the Need for Bacterial Plating
✍ Scribed by Maryam M. Matin; David P. Hornby
- Book ID
- 102561908
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 257 KB
- Volume
- 278
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
We describe here the construction of a plasmid that combines positive selection with tetracycline resistance. The vector comprises a modified version of the gene encoding the cytosine-specific DNA methyltransferase MspI and a modified form of the pBR322 tetA(C) gene. The combination of these two genes facilitates the selection of recombinant plasmids in broth cultures, thereby eliminating the need for bacterial plating.