A polynucleotide helix containing polinosinic acid and protoated polycytidylic acid

✍ Scribed by Giovanni Giannoni; Alexander Rich

• Book ID: 102760144
• Publisher: Wiley (John Wiley & Sons)
• Year: 1964
• Tongue: English
• Weight: 696 KB
• Volume: 2
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1964.360020502

✦ Synopsis

Titration studies of the 1 : 1 helical polynucleotide complex of polyinosinic acid and polycytidylic acid reveal that these two polymers form a complex when the polycytidylic acid is protonated near p H 3. Sedimentation studies show that the protonated complex also haa a 1 : 1 stoichiometry. However, the protonated complex is more stable than the unprotonated neutral complex as judged by its thermal stability. The molecular structure of the protonated complex is not the same as the neutral form. Study of the kinetics of the reaction of the protonated complex with formaldehyde suggests that the amino group of cytosine is involved in hydrogen bonds which hold the polynucleotide strands together.