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A polymerase chain reaction-based method for cloning novel members of a gene family using a combination of degenerate and inhibitory primers

✍ Scribed by Sumiko Kunihiro; Yuichi Kawanishi; Motoaki Sano; Kimitoshi Naito; Yukio Matsuura; Yoshio Tateno; Takashi Gojobori; Youhei Yamagata; Keietsu Abe; Masayuki Machida


Book ID
117520849
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
324 KB
Volume
289
Category
Article
ISSN
0378-1119

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A Polymerase Chain Reaction-Based Method
✍ M.J. Morales; D.I. Gottlieb πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 583 KB

Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt