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A polymerase chain reaction assay for the detection and quantitation of cytokine gene expression in small numbers of cells

✍ Scribed by Simon R. Carding; Dandan Lu; Kim Bottomly

Book ID: 118902563
Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 559 KB
Volume: 151
Category: Article
ISSN: 0022-1759
DOI: 10.1016/0022-1759(92)90128-g

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Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt