This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his(6)-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co(2+) with iminodiacetate (IDA) as the chelating ligand, beta-glucuronid
β¦ LIBER β¦
A poly(2-hydroxyethyl methacrylate)-based immobilized metal affinity chromatography adsorbent for protein purification
β Scribed by Che-Liang Liou; Yi-Chuan Chen; Sung-Chyr Lin
- Book ID
- 108161367
- Publisher
- Chinese Electronic Periodical Services
- Year
- 2008
- Tongue
- English
- Weight
- 886 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0368-1653
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