✦ LIBER ✦
A plate assay for the detection of organophosphonate mineralization by environmental bacteria, and its modification as an activity stain for identification of the carbon — phosphorus bond cleavage enzyme phosphonoacetate hydrolase
✍ Scribed by John W. McGrath; John P. Quinn
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 384 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0951-208X
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✦ Synopsis
A replica plate screening tcchniquc.bascd on the acid molybdatc assay for detection of phosphate.has been dcvclopcd to permit the dctcction of microorganisms capable of mincrali/.ing organophosphonates. The method was filrther adapted as the basis of an activity stain for the detection of the carbon -phosphorus bond clcavagc cnzymc phosphonoacctatc hydrolasc in PAGE gels.