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A plasmid system for high-level expression and in vitro processing of recombinant proteins

✍ Scribed by Pohlner, Johannes; Krämer, Joachim; Meyer, Thomas F.


Book ID
123048486
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
858 KB
Volume
130
Category
Article
ISSN
0378-1119

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A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase (D. B. Smith and K. S. Johnson, 1988, Gene 67, 31-40). Expressed fusion proteins are purified from bacterial extracts by glutathione-agarose affinity chromat