A Plasmid Purification Scheme for Characterization of Small Fragments Cloned into Vectors

Molecular cloning of a cDNA for a small

Molecular cloning of a cDNA for a small GTP binding protein, BRho, from the embryo of Bombyx mori and its characterization after expression and purification

✍ Tomohide Uno; Ai Nakasuji; Wajiro Hara; Yasuo Aizono 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB 👁 2 views

A cDNA clone encoding a small GTP binding protein (BRho) was isolated from an embryonic cDNA library of Bombyx mori that encoded a polypeptide with 202 amino acids sharing 60-80% similarity with the Rho1 family of GTP binding proteins. The effector site and one of the guanine nucleotide binding site