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A plasmid in the archaebacterium Methanobacterium thermoautotrophicum

✍ Scribed by Meile, Leo ;Kiener, Andreas ;Leisinger, Thomas

Publisher: Springer
Year: 1983
Tongue: English
Weight: 557 KB
Volume: 191
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425766

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✦ Synopsis

The archaebacterium Methanobacterium thermoautotrophicum Marburg (DSM 2133) was found to contain a plasmid (pME2001) in covalently closed circular form. It was isolated by CsCl gradient centrifugation of total DNA in the presence of ethidium bromide. Multimers up to the hexamer were observed upon agarose gel electrophoresis and electron microscopy of a purified plasmid preparation. A restriction map was constructed. The length of plasmid pME2001 was determined to be approximately 4,500 bp. Southern hybridization of plasmid DNA to DNA extracted from Methanobacterium thermoautotrophicum delta H (DSM1053) revealed the presence of a plasmid with homologous sequences in the delta H strain.

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