A physical standard of fibrinogen: Measurement of the elastic modulus of dilute fibrin gels with a new elastometer

✍ Scribed by Marcus E. Carr; Linus L. Shen; Jan Hermans

• Book ID: 102982583
• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 635 KB
• Volume: 72
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90522-4

✦ Synopsis

We describe the construction and use of an inexpensive and easy to use Couette elastometer with buoyant inner cylinder for measurements with dilute gels. With this instrument we have performed measurements of the static elasticity on fibrin gels at concentrations between 0.002 and I mg/ml with elastic moduli between 0.02 and 100 dyne/cm*. Me~urements with gels with lower elastic moduli are feasible; however, at lower fibrin concentrations no gelation is observed. The effects on the modulus of varying the concentration of calcium ion, of crosslinking of fibrin by Factor XII&, and of digestion of fibrinogen by plasmin have all recently been determined, The modulus is found to be a much more sensitive indication of damage done to fibrinogen than is the "clottability."

Consequently, we propose that the elastic shear modulus of dilute fibrin gels measured with this instrument be used as a standard for the characterization of fibrinogen samples. 202