The Ag stainability of the nucleolus organizer region (NOR) was studied in the acrocentric chromosomes identified by Q banding of cultured lymphocytes in 41 karyotypically normal persons (33 males and 8 females) originating from southeast Estonia. The data obtained are compared with those establishe
A photometric method for quantifying the polymorphisms in human acrocentric chromosomes
β Scribed by W. Schnedl; U. Roscher; R. Czaker
- Book ID
- 104753978
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 333 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0340-6717
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β¦ Synopsis
Photometric measurements on photomicrographs of quinacrine mustard--stained metaphase chromosomes, processed by a reverse developing procedure provide quantitative data on the polymorphisms of human acrocentric chromosomes. The method described is intended for population studies. The method is easily reproducible, allowing comparisons of data obtained by different laboratories.
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Fifteen unrelated individuals were found among the patients of the Cytogenetics Laboratory who possessed multiple-satellited marker chromosomes (14 with double satellites and 1 with triple satellites). Cytogenetic analysis was carried out by means of a conventional staining method and also by R, C,
One hundred normal Caucasians were studied by the RFA technique to estimate the frequencies of size variation of the short arm of acrocentric chromosomes. Each size variation was classified into one of five levels. The most frequent size level(code) was 3; therefore, this was regarded as the 'averag