A photochemical method for the surface modification of poly(etherurethanes) with phosphorylcholine-containing compounds to improve hemocompatibility
✍ Scribed by van der Heiden, Arthur P. ;Goebbels, Dani�lle ;Pijpers, A. Paul ;Koole, Leo H.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 179 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0021-9304
No coin nor oath required. For personal study only.
✦ Synopsis
Phosphorylcholine groups attached to polymer thrombin generation assays and platelet adhesion tests. In surfaces are known to improve hemocompatibility. A photo-thrombin generation assays the clotting time of platelet-rich chemical method is presented to couple phosphorylcholine-plasma in contact with the polymer surface is determined. containing aryl azides to poly(etherurethane) surfaces Clotting times were clearly prolonged for the modified sur-(PEUs). Two aryl azides that consist of a photoactivatable 4-faces. Surfaces modified with compound 2 showed slightly azidobenzoyl group, a short spacer chain, and a phosphoryl-higher clotting times than those modified with compound choline endgroup were synthesized. The two compounds 1. Repeated surface modification with compound 2 further differ only in the type of spacer used: triethylene glycol for increased the clotting time. For the tested surfaces an increase compound 1 and hexanediol for compound 2. These com-in the clotting time corresponds to an increase in the concenpounds were physically adsorbed to PEU surfaces. Upon UV tration of phosphorylcholine groups at the surface (as meairradiation, reactive intermediates are formed that react with sured by ESCA and contact angle). Platelet adhesion studies nucleophilic groups on the polymer surface. The modified with scanning electron microscopy demonstrated that fewer surfaces showed decreased underwater contact angles, indi-platelets (showing less activation) adhered to the modified cating that hydrophilic phosphorylcholine groups are pres-surfaces than to the unmodified polyurethane. © 1997 John ent at the surface. ESCA measurements showed the presence Wiley & Sons, Inc., J Biomed Mater Res, 37, 282-290, 1997. of phosphorus and positively charged nitrogen atoms in the outermost polymer layers (analyzed depth about 50 A ˚), Key words: phosphorylcholine; surface modification; aryl which is a strong indication of the presence of phosphoryl-azides; polyurethanes choline groups. Hemocompatibility in vitro was tested with