Cloning of the structural gene for oroti
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Buxton, F. P. ;Radford, A.
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Article
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1983
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Springer
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English
β 348 KB
A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'