A permselective-membrane electrode for the electrochemical study of redox proteins. Application to cytochrome c552 from Thiobacillus ferrooxidans
✍ Scribed by Jean Haladjian; Pierre Bianco; Frédéric Nunzi; Mireille Bruschi
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 482 KB
- Volume
- 289
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A simple and effective procedure for electrode construction, based on casting a permselective membrane onto a gold electrode surface is described. A redox protein solution is entrapped between the pennselective membrane and the electrode surface. The so-mounted permselective-membrane electrode placed in an electrolyte solution is used to study horse heart cytochrome c as a test system. The measured formal potential and the pH dependence profile are in accordance with the literature data. The permselective-membrane electrode is also used to investigate the electrochemistry of cytochrome cs5r from 'Z?aiobwillusferrooxidons and the pH dependence of its formal potential. The performance of this electrode makes it very attractive for studying redox proteins present in limited amounts only. Key worak Permselective-membrane electrode; Cytochrome c; Cytcchrome cs5*; Thiobacillus ferroo_Gdans 1. IntroductioIl