✦ LIBER ✦
A perfusion system developed for 31p NMR study of melanoma cells at tissue-like density
✍ Scribed by Margaret M. Minichiello; Daniel M. Albert; Nancy H. Kolodny; Moon-Soo Lee; Joseph L. Craft
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 875 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0740-3194
No coin nor oath required. For personal study only.
✦ Synopsis
A perfusion culture system has been developed for 31P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage-dependent cells grew to tissue-like density.