A peptide-model for the heparin-binding property of pseudorabies virus glycoprotein III

✍ Scribed by Dirk Sawitzky; Andrea Voigt; Karl-Otto Habermehl

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 505 KB
• Volume: 182
• Category: Article
• ISSN: 0300-8584
• DOI: 10.1007/bf00191944

✦ Synopsis

The pseudorabies virus glycoprotein III (PrV-glII) has been identified previously as the major viral component binding to a heparin-like receptor on the surface of target cells. The amino acid sequence of glII contains three regions corresponding to consensus sequences for heparin binding. A synthetic peptide corresponding to amino acids 134 to 141 of PrV-glII bound heparin in a dot blot assay. In contrast, a synthetic peptide derived from amino acids 290-299 of PrV-gIII did not bind heparin. We therefore conclude that the region containing amino acid 134-141 is involved in binding to the heparin-like cellular receptor.