A pentapeptide model for an early folding step in the refolding of staphylococcal nuclease: The role of its turn propensity
✍ Scribed by V. Ramakrishna; Y. U. Sasidhar
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1997
- Tongue
- English
- Weight
- 830 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Refolding of Staphylococcal Nuclease: The Role of Its Turn Propensity
Recen [l! [ht', mcharrge [echnique. Vfany oi'the rnidue.v that showed significant projection are Ioca[ed in (isheet regions,t~.v[rll~[llrt~,
if fiwmed b! .s[rurrd.2 and 3 (} fsheet 2 connected b!' [he .vequeme ~-Y KGQP3) in u [~pc 1'rwer.sc (urn confi)rtnution ni(h a 4 + I h?zlrogen bonding be[wven Q30 Iv]! and Y27 <'=0. 14'ehave {argeied the conlinmaiiona[ characterization of the peptide model .4c-YK(iQP-N!ll vii[h 'Ii [)ttj-di~~l(~n.vi~)nal ntnr [<whnique.rin aqueous solution ~~i(ha vim~'[o assessing it.~propen vi[~(c) sample turn con/imnat imral,jin-m.~and thus ini[ia[e [h~',[imna[ion of/3-hairpin \trtu[urc Bawd
upon [he observed clan (i, i q 1), clan (i, i + 3), and ctnn(i, i + 1) nuclear Overhauser e~li 'ct connec(i~'i[ies, ternpera[ure [we firienis ,fi~ramide proton.~and conjixma[ional anal] .vi,~M i[h quantum mechanical perturhative conJigura[ion in(erac{ion tn'er loca[ized orbital.s method, we conclude thal [he model peptide samples turn conli)rrnalional /irons w'i[hreduced c~jn/iwmational eruropy. Wk .Niggest [hat [hc turn can rrucleale thejiwrna[ icm of the (ihairpin .struct ure in [he r<jiidirrg o~'nucleu,sc Observu[iotr c!j't urn propen.vit~,lijr this wquerrce is con.si.rtcnt Bit h [ho ji)lding mechanism oj 'the Greek k('? mot~(pre,sent in S[aph~lococcal rruclea.w) proposed in [he literature '.