A PCR-based strategy for Dombrock screening in Brazilian blood donors reveals a novel allele: the DO*A-WL
✍ Scribed by Wilson Baleotti Jr.; Rodrigo Buzinaro Suzuki; Milena Polotto; Marcelo Ortega Ruiz; Antonio Fabron Jr.; Lilian Castilho
- Book ID
- 102311733
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 182 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Background: Determination of the molecular basis underlying the antigens in the Dombrock blood group system has shown various rearrangements between the alleles associated with DO^*^A and DO^*^B. Based on this, we employed a PCR‐based strategy to screen DO alleles (DO^*^A, DO^*^B, HY^*^1, HY^*^2 and JO) in Brazilians. Methods: We tested DNA of 278 Brazilian blood donors by PCR‐RFLP on plates of 96 wells to determine the 793A/G (DO^*^A/DO^*^B), 323G/T (HY), 350C/T (JO) and 898C/G (HY^*^1/HY^*^2) single nucletide polymorphisms. In order to confirm the results sequence analysis was also performed. Results: When samples of these donors were analyzed, a novel allele combination, the DO^*^A allele (793A and 323G) associated with 898G was identified and designated as DO^*^A‐WL allele. This new allele encoding 300Val is the same as HY^*^1 at nucleotide 898 on the molecular background of DO^*^A. Among the 556 alleles analyzed by PCR‐RFLP, 3 were DO^*^A‐WL and 78 were DO^*^B‐WL. This represents an overall frequency of 0.5% for DO^*^A‐WL and 14% for DO^*^B‐WL across the population studied. Conclusion: Molecular screening of Brazilians revealed one novel allele, the DO^*^A‐WL. Our data highlight the importance of testing a cohort of different populations to determine DO haplotypes and to establish reliable genotyping tests for predicting Do^a^/Do^b^ status. J. Clin. Lab. Anal. 25:79–82, 2011. © 2011 Wiley‐Liss, Inc.