A parid method of determining peroxidase activity
โ Scribed by M. Srinivasan; S. Ramaswamy; M. Sreenivasaya
- Book ID
- 112979772
- Publisher
- Springer India
- Year
- 1941
- Weight
- 477 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0370-0097
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๐ SIMILAR VOLUMES
A sensitive fluorimetric method with guaiacol as the hydrogen donor for peroxidase activity is described. The gist of this method is measurement of the fluorescence (excitation, 300 nm; emission, 340 nm) in cyclohexane of mainly a guaiacol dimer which forms in the early phase of the color formation.
A peroxidase specific zymogram staining procedure has been designed which not only locates peroxidase isozymes which have been resolved electrophoretically, but. simultaneously estimates the relative activity of each peroxidase isozyme in the system under study. This quantitative assay of individual