A nude mouse wilms' tumor line (KCMC-WT-1) derived from an aniridia patient with monoalleleic partial deletion of chromosome 11p
✍ Scribed by Yoji Nagashima; Hirokazu Nishihira; Yohei Miyagi; Yukichi Tanaka; Yoshiro Sasaki; Toshiji Nishi; Kiyoshi Imaizumi; Ichiro Aoki; Kazuaki Misugi
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 623 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
BACKGROUND.
A candidate tumor suppressor gene, WT-1, is believed to have an important role in the pathogenesis of Wilms' tumor, especially that occurring in patients with congenital aniridia.
METHODS.
To obtain a stable tumor line to work with, Wilms' tumor tissue was serially transplanted in athymic nude mice. Biopsied Wilms' tumor tissue, derived from an aniridia patient, was transplanted subcutaneously to an athymic nude mouse, and then transplanted serially. Histopathologic and molecular biologic studies were performed on the xenotransplants.