✦ LIBER ✦

A nuclear cAMP binding protein in retinoic acid-treated HL-60 cells

✍ Scribed by Robert C. Briggs; Susan B. Casey

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 438 KB
Volume: 136
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041360127

No coin nor oath required. For personal study only.

✦ Synopsis

A cAMP binding protein was detected in HL-60 cells using photoaffinity labeling with 8-azido ["PIcAMP. The binding protein was found i n a 0.35 M NaCl nuclear protein extract from untreated HL-60 cells and from the HL-60 cells induced to mature with retinoic acid. While the quantity of the cAMP binding protein did not change following the induced differentiation, a second form of the subunit, altered in charge, was present at 3 and 5 days after retinoic acid treatment. The findings indicate that the regulatory subunit of the type II CAMP-dependent protein kinase could be involved in nuclear functions associated with human myeloid cell differentiation.

📜 SIMILAR VOLUMES

Manganese enhances phosphorylation of a

Manganese enhances phosphorylation of a 47 kD protein in retinoic acid-induced HL-60 cells

✍ Karol R. Smith; Susan S. Percival 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 328 KB 👁 1 views

We previously observed that HL-60 cells treated with manganese (Mn) during differentiation displayed an enhanced oxidative burst. Since a Mn-dependent kinase has been identified and phosphorylation is involved in burst activation, the objective of this research was to identify proteins in retinoic a

Effect of retinoic acid on the phosphory

Effect of retinoic acid on the phosphorylation of endogenous proteins in HL-60 cells

✍ Terry N. Spearman; Joseph A. Fontana; Fred R. Butcher; John P. Durham 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 990 KB

HL-60 cells were incubated with ["Pl-P, in order to label endogenous phosphoproteins in situ. These were then resolved via two-dimensional electrophoresis and autoradiograms were made from the gels. A comparison of autoradiograms made from rctinoic-acid-differentiated cells with those made from cont

Expression of cytoplasmic retinoic acid-

Expression of cytoplasmic retinoic acid-binding proteins and nuclear receptors in squamous-cell carcinomas in vitro

✍ Jeanette Kley; Wolfgang Hoffmann; Michael Bamberg; H. Peter Rodemann 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 50 KB 👁 2 views

Retinoids have been described to be potent modulators of cell proliferation, differentiation and apoptosis in various types of squamous-cell carcinoma (SCC) and myeloid cancer cells in vitro (Bollag and Holdener, 1992;Lotan et al., 1995). It has also been demonstrated that treatment of cells with re

Erratum: Smith, Karol R, Percival, Susan

Erratum: Smith, Karol R, Percival, Susan S. (1998): Manganese enhances phosphorylation of a 47 kD protein in retinoic acid-induced HL-60 cells. J. Cell. Physiol. 176:188-195

✍ Karol R. Smith; Susan S. Percival 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 43 KB 👁 1 views

Immunolocalization of cellular retinoic

Immunolocalization of cellular retinoic acid binding protein to müller cells and/or a subpopulation of GABA-positive amacrine cells in retinas of different species

✍ Ann H. Milam; A. Margreet De Leeuw; Vinod P. Gaur; John C. Saari 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 922 KB

Identification of a Cis-acting element i

Identification of a Cis-acting element in the rat α-fetoprotein gene and its specific binding proteins in F9 cells during retinoic acid-induced differentiation

✍ Hongmin Chen; Jing-Ming Dong; Yi Liu; Jen-Fu Chiu 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 209 KB 👁 1 views

Mouse F9 embryonic teratocarcinoma stem cells can be induced to differentiate into visceral endoderm. Following retinoic acid (RA) treatment, alpha-fetoprotein (AFP), a differentiation marker, is expressed and secreted. The mechanism by which RA regulates AFP expression during differentiation is not