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A Novel Temperature-Responsive Polymer as a Gene Vector

✍ Scribed by Yongzheng Ma; Sen Hou; Bin Ji; Yang Yao; Xizeng Feng

Book ID
102474283
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
360 KB
Volume
10
Category
Article
ISSN
1616-5187

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✦ Synopsis

Abstract

A temperature‐responsive polymer poly{2‐(dimethylamino)ethyl methacrylate‐co‐[cis‐butenedioic anhydride‐poly[(N‐isopropylacrylamide)‐co‐(butyl methacrylate)]]} (PDMNIB) was synthesized by free radical polymerization. The polymer had a significant temperature‐responsive behavior with a lower critical solution temperature (LCST) at 20 °C. Gel retardation assay showed that PDMNIB could efficiently interact with DNA. Dynamic light scattering (DLS) and zeta potential measurement indicated that the average sizes and the surface electric charges of the PDMNIB/DNA complexes could be changed by temperature. Due to the thermosensitive interaction between PDMNIB and DNA, the gene transfection efficiency of PDMNIB could be improved by temperature.

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