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A novel technique to specifically analyze the secretome of cells and tissues

✍ Scribed by Hannes Zwickl; Elisabeth Traxler; Stefan Staettner; Wolfram Parzefall; Bettina Grasl-Kraupp; Josef Karner; Rolf Schulte-Hermann; Christopher Gerner


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
892 KB
Volume
26
Category
Article
ISSN
0173-0835

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Two-photon multicolor fluorescence in situ hybridisation (FISH) is presented as an advanced single molecule detection technique. Based on a two-photon excitation process it enables pinhole-free 3D laser scanning microscopy. Nonresonant two-photon absorption in a subfemtoliter excitation volume was r