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A novel strategy to identify maternal and paternal inheritance in the mouse

✍ Scribed by Dr. Irmgard Römer; Peter Jungblut; Wolf Reik; Albrecht Otto; Joachim Klose

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
960 KB
Volume
16
Category
Article
ISSN
0173-0835

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✦ Synopsis

A novel strategy to identify maternal and paternal inheritance in the mouse

A novel strategy for identifying proteins which reveal maternal or paternal inheritance in the mouse is presented. Using two-dimensional electrophoresis we investigated protein expression patterns of adult liver and different embryonic and extraembryonic tissue in C57BL16Crl and in DBA/2Crl mice, as well as in their reciprocal hybrids. We found three groups of protein spots which showed maternal or paternal inheritance of quantitative variations. These proteins were characterized by N-terminal or internal amino acid sequencing, by determination of the amino acid composition, by glycoprotein staining and RNA expression analysis. The three proteins identified were: a-enolase, cyclophilin and (3-group hemoglobins. The parental effects observed for a-enolase and cyclophilin were found to be due to parent-specific posttranslational modifications of these proteins. For the fi-group hemoglobins our results suggested parental effects on the transcriptional level.

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