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A novel site for streptomycin resistance in the “530 loop” of chloroplast 16S ribosomal RNA

✍ Scribed by Hillel Fromm; Esra Galun; Marvin Edelman

Publisher
Springer
Year
1989
Tongue
English
Weight
722 KB
Volume
12
Category
Article
ISSN
0167-4412

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✦ Synopsis

The chloroplast gene for 16S rRNA was cloned from two maternally inherited streptomycin-resistant mutants of Nicotiana differing in degree of resistance at the whole plant and isolated chloroplast level. A single-nucleotide change in the 16S rRNA gene was detected for each mutant: a C to T transition at nucleotide 860 (Escherichia coli coordinate C912) which is an often mutated site, and a novel transition of C to T at nucleotide 472 (E. coli coordinate C525). The novel mutation is located in the phylogenetically conserved "530 loop".

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Mapping of chloroplast mutations conferring resistance to antibiotics in Chlamydomonas: Evidence for a novel site of streptomycin resistance in the small subunit rRNA
✍ Gauthier, Antonin ;Turmel, Monique ;Lemieux, Claude 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 961 KB

A major obstacle to our understanding of the mechanisms governing the inheritance, recombination and segregation of chloroplast genes in Chlamydomonas is that the majority of antibiotic resistance mutations that have been used to gain insights into such mechanisms have not been physically localized