A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide)
✍ Scribed by Okano, Teruo ;Yamada, Noriko ;Sakai, Hideaki ;Sakurai, Yasuhisa
- Book ID
- 102874216
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 1022 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Poly(N-isopropyl acrylamide) (PIPAAm) demonstrated a fully expanded chain conformation below 32°C and a collapsed, compact conformation at high temperatures. This unique temperature responsive polymer was grafted onto surfaces of commercial polystyrene dishes and used as temperature switches for creating hydrophilic surfaces below 32°C and hydrophobic surfaces above 32°C. Cell attachment and the growth of bovine endothelial cells and rat hepatocytes on PIPAAm-grafted surfaces at 37°C demonstrated similar behavior to the commercialized culture dishes. Both cell types were observed to detach from the PIPAAm-grafted surface simply by reducing the temperature below the polymer transition temperature (collapse). Cells recovered by this method maintained substrate adhesivity, growth, and secretion activities nearly identical to those found in primary cultured cells in contrast to the compromised function found in cultured cells damaged by trypsinization. These resuIts provide strong evidence that PIPAAm-grafted surfaces, as thermal switches are very effective for reversing cell attachment and detachment without cell damage. Properties of cell culture surfaces can be readily transformed by this technique reversibly into hydrophilic and hydrophobic coatings of PIPAAm-grafted polymers.