A novel polymorphism of human complement component C3 detected by means of a monoclonal antibody

✍ Scribed by Claus Koch; Niels Behrendt

• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 401 KB
• Volume: 23
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00398796

✦ Synopsis

A mouse monoclonal antibody, HAV 4-1, obtained after immunization of a BALB/c mouse with purified C3F, detected a novel genetic polymorphism of human complement component C3 in a simple immunoblotting system. The frequency of HAV 4-1-positive genes was 20.1%. Reactivity of HAV 4-1 was closely related to C3F, but certain individuals with the C3F allele did not react with HAV 4-1. Conversely, certain C3S homozygous individuals did react with HAV 4-1. The polymorphism detected by this monoclonal antibody is therefore different from the previously described polymorphism based on charge differences.