A Novel Polybrene/Chondroitin Sulfate C Double Coated Capillary and Its Application in Capillary Electrophoresis

✍ Scribed by Ying-Xiang Du; Susumu Honda; Atsushi Taga; Wen-Ying Liu; Shigeo Suzuki

• Book ID: 102803125
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 623 KB
• Volume: 20
• Category: Article
• ISSN: 0256-7660
• DOI: 10.1002/cjoc.20020201218

✦ Synopsis

Abstract

A new capillary coated by double polymer, polybrene/chondroitin sulfate C (P/CC), was developed using a simple procedure. The P/CC double coated capillary showed long lifetime, strong chemical stability and good reproducibility. It endured during more than 100 replicated analyses and was also tolerant to HCl (1 mol/L), NaOH (0.01 mol/L), CH~3~OH and CH~3~CN. The P/CC double coated capillary can be applied to basic drug analyses. The adsorption of basic drugs to the capillary wall was suppressed and the peak tailing greatly decreased. The use of the P/CC double coated capillary allowed excellent separation of the enantiomers of some basic drugs by using chondroitin sulfate C as the chiral selector, and the peak symmetry of basic drugs was further improved under these conditions.