A novel photometric glucose biosensor based on decolorizing of silver nanoparticles

✍ Scribed by J. Tashkhourian; M.R. Hormozi-Nezhad; J. Khodaveisi; R. Dashti

• Publisher: Elsevier Science
• Year: 2011
• Tongue: English
• Weight: 428 KB
• Volume: 158
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/j.snb.2011.06.002

✦ Synopsis

A novel glucose biosensor based on chromophore (silver nanoparticles) decolorizing for the photometric determination of glucose was developed. Silver nanoparticles are directly synthesized in the sol-gel matrix by a one-step method based on the reduction of the inorganic precursor AgNO 3 and were used for the preparation, characterization and calibration of a highly sensitive and cost-effective localized surface plasmon resonance-based glucose biosensor. In the presence of glucose oxidase (GOx) and due to the enzyme-substrate (glucose) reaction, H 2 O 2 was produced and silver nanoparticles in the sol-gel glass have the ability for the decomposition of hydrogen peroxide. Due to the degradation of silver nanoparticles a remarkable change in the localized surface plasmon resonance absorbance strength could be observed which have been monitored as a suitable signal for determination of substrate concentration. Beer's law is obeyed in the range from 50 to 800 mg/L glucose and the limit of detection is 23 mg/L. The proposed optical biosensor has been successfully applied to the determination of glucose in various real samples.