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A novel nuclear protein which binds to Gγ and Aγ globin promoters and modulates hemoglobin synthesis in K562 cells

✍ Scribed by B. S. Baliga; Karen Phillips; Zarrintaj Aliabadi; Vipul N. Mankad

Book ID: 102304098
Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 471 KB
Volume: 49
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240490409

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✦ Synopsis

Nuclear extract of human erythroleukemic cell line KjG2 contains a 70 kDa protein which is gradually reduced when cells are induced to express globin genes by 25 FM hemin. When globin synthesis was inhibited by cycloheximide (100 pg/ml) or Actinomycin D (1 Fg/ml), the disappearance of this protein was prevented. The 70 kDa nuclear protein exhibited strong binding to Cy and Ay globin promoters but not to P-globin promoter. This suggests that this 70 kDa nuclear protein may be involved in the regulation of fetal globin gene expression.

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