A novel neuropeptide precursor gene is expressed in the terrestrial snail central nervous system by a group of neurons that control mating behavior
✍ Scribed by Poteryaev, Dmitry A. ;Zakharov, Igor S. ;Balaban, Pavel M. ;Belyavsky, Alexander V.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 261 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
We report the isolation of a cDNA its L-Phe-containing stereoisomer. Injection of synclone encoding a neuropeptide precursor named prethetic D-GFAD in nanomolar concentrations into in-proGFAD from the central nervous system (CNS) of tact animals caused an increase of the heartbeat rate the snail Helix lucorum. Analysis of the expression of and opening of the genital atrium. In preparations this gene shows that it is neurospecific and expressed containing CNS with intact innervation of reproducin several groups of CNS neurons. Most notable is the tive organs, bath application of D-GFAD caused exexpression of preproGFAD gene in the right mesotensive movements of the penis but not of other reprocerebrum, where the neurons controlling mating beductive organs. Intracellular activation of individual havior are located. The expression in this particular neurons expressing the preproGFAD gene also elicited region is observed in adult animals but not in juvenile penis movements. D-GFAD also suppressed activity ones. The preprohormone is 108 amino acids long and of neurons modulating feeding behavior. Our data contains a hydrophobic leader peptide and eight Lystherefore indicate that the preproGFAD gene encodes Arg recognition sites for endoproteolysis. The post-
the precursor of a neuropeptide that participates in translational processing of the prohormone may lead the regulation of male mating behavior. ᭧ 1998 John to the generation of seven tetrapeptides, Gly-Phe-Ala-