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A novel mutation that affects utilization of galactose inSaccharomyces cerevisiae

โœ Scribed by Yasuhisa Nogi; Toshio Fukasawa

Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
512 KB
Volume
2
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

A novel type of regulatory mutation for galacrose metabolism in Saccharomyees cerevisiae is described. The mutation named galll was recessive, non-alMic to GAL4, GALSO, GAL2, or GAL3, and unlinked to the gene cluster of GALl, GALIO, and GilL Z It caused a 'coordinate' reduction of galactokinase, galactose-l-P uridylyl transferase, and UDP-glucose 4-epimerase by a factor of more than 5, rendering the mutant cells galactose-nonfermenting. The effect of the mutation was manifested not only in cells grown on galactose but also in cells constitutively synthesizing the galactose-metabofizing enzymes.

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