A novel monoclonal antibody, C41, reveals IL-13Rα1 expression by murine germinal center B cells and follicular dendritic cells
✍ Scribed by Johanne Poudrier; Pierre Graber; Suzanne Herren; Claude Berney; Denise Gretener; Marie H. Kosco-Vilbois; Jean-François Gauchat
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 219 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Responsiveness to IL-13 involves at least two chains, IL-4R § and IL-13R § 1. Although mouse B cells express IL-4R § , little is known about their expression of IL-13R § chains. To investigate this topic further, we have generated a monoclonal antibody (C41) specific for murine IL-13R § 1. Using C41, IL-13R § 1 expression was detected on germinal center (GC) B cells by flow cytometry and immunohistochemistry. In addition, IL-13R § 1 was observed on follicular dendritic cells, but not interdigitating dendritic cells in the T cell areas. Furthermore, resting B cells also expressed IL-13R § 1, and in the presence of IL-13 produced increased amounts of IgM in response to in vitro CD40 stimulation. However, C41 was unable to neutralize this bioactivity. The distribution of IL-13R § 1 on murine B cells and during GC reactions suggests a role for IL-13 during B cell differentiation.